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. 2021 Jan 29;2021:8897906. doi: 10.1155/2021/8897906

Figure 3.

Figure 3

linc01184 directly targeted miR-331 and acted as ceRNA of miR-331 in CRC cells. (a) The prediction of the binding region between linc01184 and miR-331; (b) the sequence of wild-type (WT) and mutant (MUT) linc01184 luciferase reporters; (c) dual-luciferase reporter assay revealed that linc01184 directly targeted miR-331, ∗p < 0.05 vs. luci-linc01184-MUT or negative control (NC) mimic; (d) the analysis of enrichment between linc01184 and miR-331 probes using the RNA pull-down assay, ∗p < 0.05 vs. control probe or miR-331 mutant (MUT) probe; (e) linc01184 expression in transfected HCT-116 cells, ∗p < 0.05 vs. empty vector, #p < 0.05 vs. 0.5 μg/mL pCDNA-linc01184; (f) miR-331 expression in transfected HCT-116 cell, ∗∗p < 0.01 vs. empty vector or scramble control.