Figure 4.

The change of HER2 protein level expression and cell apoptosis in HCT-116 cells under various conditions. (a) Western blot showed that linc01184 overexpression (pCDNA-linc01184 transfection) or miR-331 downregulation (miR-331 inhibitor transfection) significantly enhanced HER2 expression, which was abolished by siRNA; however, the effect of siRNA on HER2 expression can be antagonized by miR-331 inhibitor; (b) fold change of HER2 was significantly increased by linc01184 overexpression or miR-331 downregulation, which was opposite to siRNA transfection; (c) linc01184 overexpression or miR-331 downregulation significantly suppressed CRC cell apoptosis. ^∗p < 0.05 vs. scramble control.