Skip to main content
. 2021 Jan 20;41(1):100–106. [Article in Chinese] doi: 10.12122/j.issn.1673-4254.2021.01.14

3.

3

20(S)-Rg3通过抑制DNMT3A介导的DNA甲基化而上调VHL

20(S)-Rg3 upregulates VHL via suppressing DNMT3A-mediated methylation in the promoter region of VHL gene. A: Real-time PCR results show that 20(S)-Rg3 treatment decreases DNMT3A mRNA level and increases DNMT3B mRNA level without affecting DNMT1 mRNA in the cells; B: Western blotting results show that 20(S)-Rg3 treatment decreases DNMT3A and increases DNMT3B protein expressions without affecting DNMT1 expression in the cells; C: Real-time PCR shows reduced VHL mRNA level in 20(S)-Rg3-treated cells overexpressing DNMT3A; D: Western blotting results show lowered VHL protein expression in 20(S)-Rg3-treated cells overexpressing DNMT3A; E: MSP results show increased methylation level in the promoter region of VHL gene in 20(S)-Rg3-treated cells overexpressing DNMT3A. U=Unmethylated. M= Methylated. Ns : non-significant; *P < 0.05.