1.

序列信息

Primer sequences

Genes	Sequences (5'-3')
Real-time PCR Primer
VHL	F: GCAGGCGTCGAAGAGTACG
	R:CGGACTGCGATTGCAGAAGA
DNMT3A	F: TATTGATGAGCGCACAAGAGAGC
	R:GGGTGTTCCAGGGTAACATTGAG
DNMT3B	F: GACTCGAAGACGCACAGCTG
	R:CTCGGTCTTTGCCGTTGTTATA
DNMT1	F: GTTCTTCCTCCTGGAGAATGTC
	R: GGGCCACGCCGTACTGTTGCAG
β-actin	F: TCCCTGGAGAAGAGCTACGA
	R: AGCACTGTGTTGGCGTACAG
MSPPrimer
VHL methylated(M)	F: GGAGGATTATTTGAATTTAGGAGTTC
	R: TAAAACAAAATCTCACTCTATCGC
VHL unmethylated(U)	F: GGATTATTTGAATTTAGGAGTTTGA
	R: TAAAACAAAATCTCACTCTATCACC