Fig. 6.

Impact of HSP101–1 on the fertility of neo-tetraploid rice. a Expression pattern analysis of HSP101–1 in neo-tetraploid rice Huaduo 1 using qRT-PCR. b Plant morphology, panicle and pollen fertility of wild type Huaduo 1 (left) and hsp101–1 (right). c, d Comparison of seed setting and pollen fertility between wild type Huaduo 1 and hsp101–1. 2 L, 2S, 3 L, 3S, 4 L, 4S: leaf and stem at meiosis stage, bicellular pollen stage and 5 days after flowering; M, M1, M3: young panicle at the length of 0.5 cm 1 cm and 5 cm; PMAA, PMAO, MA1A, MA1O, MA2A, MA2O, SCPA, SCPO, SCP2A, SCP2O, BCPA, BCPO: anther and ovary at pre-meiotic interphase, meiosis I, meiosis II, microspore early, microspore late and bicellular pollen stages. Scale bars: 20 cm for plant, 1 cm for panicle and 100 μm for pollen