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. 2020 Mar 31;78(1):337–350. doi: 10.1007/s00018-020-03504-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — O-glycan profiles from two exemplary CRC cell lines. a O-glycan profile of the mucin-secreting well-differentiated (colon-like) cell line LS180 is characterized by high expression of sialyl Lewis x/a and Lewis x/a antigens. These antigens were found to be present on core 1, core 2 and core 4 glycan structures, both in linear and I-branched form. In contrast, b displays poorly differentiated human colon adenocarcinoma cell line DLD-1, showing an O-glycan profile which is dominated by core 2 sialylated glycans lacking any fucosylated antigens