Fig. 2.

Chromatographic separation of O-glycan isomers at m/z 1186.40 [M–H]⁻ carrying different antigens. The extracted ion chromatograms show a different retention behaviour of Lewis antigen isomers in the a LS180 cell line. Namely, core 1 carrying Lewis x antigen with α2,6-linked NeuAc linked to the core GalNAc (RT = 30.2 min), core 2 glycan carrying sialyl-Lewis x antigen on the 6 arm (RT = 37.2 min), and core 2 glycan carrying Lewis x antigen on the 6 arm (RT = 40.7 min). Additionally, the b CaCo-2 cell line illustrates the separation of core 2 blood group antigen H type 2 (RT = 52.4 min), and a core 2 mucin blood group antigen H type 3 (RT = 63.8 min)