Fig. 7.

Expression of human embryonic stem cell markers and pluripotency of control and CMT2B hiPS cells. a Immunohistochemistry for embryonic stem (ES) cell markers of CMT2B iPS cells. Bars 50 µm. b RT-PCR analysis of ES cell-marker genes in human iPS cells, human dermal fibroblast (HDF) and previously derived iPS cell as positive control. Primers used for Oct3/4, Sox2, Klf4, and c-Myc specifically detect the transcripts from the endogenous genes, but not from the retroviral transgenes. c RT-PCR for expression of retroviral transgenes in human iPS cells, HDF, and HDF 7 days after the transduction with the four retroviruses as a positive control. d Immunohistochemistry of iPS cells spontaneously differentiated show markers for the three germ layers. Bars 50 µm