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. Author manuscript; available in PMC: 2022 Feb 4.
Published in final edited form as: Mol Cell. 2020 Dec 17;81(3):614–628.e4. doi: 10.1016/j.molcel.2020.11.033

Figure 5∣. Collided ribosomes with an eRF1-bound-lead ribosome do not activate ISR but activate RQC.

Figure 5∣

A) Schematic showing how a catalytically dead mutant (GAQ) of eRF1 can be used to cause ribosome collisions at stop codons with the factor bound to the A site of the lead ribosome. B) Western-blot analysis of total proteins isolated from cells expressing wild type or catalytically dead mutant of eRF1 in the absence and presence of induction by galactose. C) Polysome profiles of lysates isolated from cells expressing the indicated-release-factor variants. Top shows profiles of untreated lysates; bottom shows those of RNase I-treated lysates.