KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti phosphor-eIF2a (Ser51) | Cell Signaling Technology | 9721S |
| rabbit anti-GCN4 | Thomas Dever lab | N/A |
| rabbit anti GCN1 | Alan Hinnebusch lab | N/A |
| mouse anti-ubiquitin HRP | Santa Cruz | sc8017 |
| mouse anti-FLAG | Sigma | F1804 |
| rabbit anti-uS4 (Rps9) | Abcam | ab117861 |
| rabbit anti-eRF1 | Rachel Green lab | N/A |
| goat anti mouse IgG HRP | Thermo Scientific | 31430 |
| goat anti rabbit IgG HRP | Thermo Scientific | 31460 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Phusion High Fidelity DNA polymerase | NEB | Cat#: M0530 |
| Ambion RNase I | Thermo Scientific | AM2294 |
| iTaq Universal SYBR Green Supermix | BIO-RAD | cat#1725121 |
| M-MuLV reverse transcriptase | NEB | cat#M0253L |
| Random hexamer | Thermo Scientific | SO142 |
| SuperSignal West Femto Maximum Sensitivity Substrate | Thermo Scientific | Cat#: 34095 |
| SuperSignal West Pico Chemiluminescent Substrate | Thermo Scientific | Cat#: 30480 |
| N-Ethylmaleimide | Millipore Sigma | Cat#: E3876 |
| Xbal | NEB | Cat#: R0145 |
| BamHI | NEB | Cat#: R0136 |
| XhoI | NEB | Cat#: R0146 |
| T4 DNA Ligase | NEB | Cat#: M0202 |
| DNA Polymerase I, Large (Klenow) Fragment | NEB | Cat#: M0210 |
| DNase I | Thermo Scientific | Cat#: 89836 |
| cycloheximide ultra pure | VWR | Cat#: 94271 |
| 4-nitroquinoline 1-oxide | Millipore Sigma | N8141-1G |
| Methyl methanesulfonate | Millipore Sigma | 129925-25G |
| Hydroxyurea | Millipore Sigma | H8627-5G |
| Phenylmethylsulfonyl fluoride | GoldBio | P-470-10 |
| anisomycin | Millipore Sigma | A5862 |
| tigecycline | Fisher Scientific | T3589-100mg |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO:GSE150790 |
| Experimental Models: Organisms/Strains | ||
| BY4741 (matA) | Dharmacon lab | N/A |
| DOM34Δ | Doma and Parker, 2006 | N/A |
| SUI2 (iosgenic strain for sui2-S52A) | This paper | N/A |
| sui2-S52A | This paper | N/A |
| SUI2, HEL2Δ | This paper | N/A |
| sui2-S52A; HEL2 Δ | This paper | N/A |
| HEL2Δ | Yan et al., 2019 | N/A |
| GCN1Δ | Dharmacon | N/A |
| GCN2Δ | This paper | N/A |
| GCN4Δ | Dharmacon lab | N/A |
| Hel2Δ; GCN2Δ | This paper | N/A |
| Rps3-FLAG | Simms et al., 2017 | N/A |
| GCN20Δ | Dharmacon lab | N/A |
| SLH1Δ | This paper | N/A |
| Oligonucleotides | ||
| DNA oligos for cloning: See Table S2 | N/A | N/A |
| Recombinant DNA | ||
| pYES2-eRF1 | This paper | N/A |
| pYES2-eRF1(GAQ) | This paper | N/A |
| Software and Algorithms | ||
| Ensembl release 76 | N/A | GCA_000146045.2 |
| STAR version 2.5.1a | Dobin et al., 2013 | 2.5.1a |
| Subread:featureCount | Liao et al., 2014 | version 1.4.6-p5 |
| Salmon | N/A | version 0.8.23 |
| RSeQC | Wang et al., 2012 | version 2.6.2 |
| Perseus | Tyanova et al., 2016 | 1.6.12 |
| Image Lab | Bio-Rad | 6.1 |
| GraphPad Prism | GraphPad | 8.4.3 |