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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Transl Res. 2020 Oct 22;229:100–114. doi: 10.1016/j.trsl.2020.10.005

Figure 1. Extracellular mucus is cytoprotective for cancer cells and imparts chemoresistance.

Figure 1.

TUNEL assay was performed on MCP and non-MCP tumor tissues, with or without OXAL (100μM) treatment, for 24h ex vivo (A) and TUNEL-positive cell were quantified; images were randomly taken of 6 different fields and analyzed using Image J software to quantify apoptosis (B). LS174T cells were coated with undiluted or diluted mucus (diluted to 10 mg/ml or 100 mg/ml in PBS), treated with OXAL (0-100μM) for 24h, following which cell viability was analyzed by MTS assay (C). In a tranwell assay, delivery of OXAL (upper chamber) to LS174T cells (lower chamber) through undiluted or diluted mucus separating the chambers was determined by quantifying intracellular platinum using AAS; the insert is a pictorial representation of the transwell setup (D). Error bars represent standard deviation (SD) from triplicate experiments (**P< .01, ***P< .001).