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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Transl Res. 2020 Oct 22;229:100–114. doi: 10.1016/j.trsl.2020.10.005

Figure 2. Extracellular mucus can be lysed with a combination of BRO and NAC.

Figure 2.

Extracellular mucus (1g) from MCP patients were treated with BRO (300 μg/ml) + NAC (250 mM) in 10 ml PBS solution or PBS alone (CTRL) at 37°C for 24h at varying pH (pH 6.5-8.5) (A). At 24h, the mucus solution from (A) was filtered and the residual mucus gel was weighed (B). Protein was extracted from the filtered solution and western blot assay for MUC2 protein was performed (C). Complete mucolysis of 1g of mucus was achieved when treated with BRO (300 μg/ml) + NAC (250mM) in 10 ml PBS (pH 8.0) at 37°C for 2h, while minimal mucolysis was achieved by BRO alone or NAC alone under the same condtions (D). Error bars represent standard deviation (SD) from triplicate experiments (**P< .01, ***P< .001).