KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| Ampicillin | Sigma | Cat# A8351 |
| Kanamycin | Fisher scientific | Cat# BP906–5 |
| Tetracycline | Fisher scientific | Cat# BP912 |
| IPTG | Zymo Research | Cat# I1001–5 |
| Tris | Fisher scientific | Cat# BP152–1 |
| NaCl | Fisher scientific | Cat# BP358–212 |
| L-Glutamate | Sigma | Cat# 49621 |
| L-Methionine | Sigma | Cat# M9625 |
| MgSO4 | Fluka | Cat# 13143 |
| DNAse | Sigma | Cat# DN25–1 |
| PMSF | Acros Organics | Cat# 215740500 |
| 2-Mercaptoethanol (BME) | Acros Organics | Cat# 125470100 |
| Ni-Affinity Resin | Takara | Cat# 635660 |
| Imidazole | Acros Organics | Cat# 301870025 |
| Thrombin | Haematologic Technologies | Cat# HCT-0020 |
| Glycerol | Fisher scientific | Cat# BP229–4 |
| MES buffer | Sigma | Cat# M2933 |
| PEG 2000 MME | Fluka | Cat# 81321 |
| Ammonium Sulfate | Fisher scientific | Cat# A702–500 |
| Glycine | Jena Biosciences | Cat# CS-507L |
| Ammonium acetate | Fisher scientific | Cat# BP326–500 |
| Sodium Acetate | Fisher scientific | Cat# S209–500 |
| Dulbecco’s Modified Eagle’s Medium | Gibco | Cat# 10566024 |
| Fetal bovine serum | Gibco | Cat# 16140071 |
| Penicillin Streptomycin Fungizone | Cytiva HyClone | Cat# SV3007901 |
| FugeneHD | Promega | Cat# E2311 |
| EGTA | Sigma | Cat# E4378 |
| Bis-Tris Propane | RPI | Cat# B78000100.0 |
| HEPES | Sigma | Cat# H3375–250G |
| D-mannitol | Fisher | Cat# M120–500 |
| Ca-Gluconate | Sigma | Cat# C8231–100G |
| Deposited Data | ||
| Coordinates of GLR3.2-S1S2-Glycine | This paper | PDB: 6VEA |
| Coordinates of GLR3.2-S1S2-Methionine | This paper | PDB: 6VE8 |
| S1S2 of GluA2 | (Armstrong and Gouaux, 2000) | PDB: 1FTJ |
| S1S2 of GluK2 | (Mayer, 2005) | PDB: 1S50 |
| S1S2 of GluN1 | (Furukawa and Gouaux, 2003) | PDB: 1PB7 |
| S1S2 of GluN2A | (Furukawa et al., 2005) | PDB: 2A5S |
| S1S2 of AvGluR1 | (Lomash et al., 2013) | PDB: 4IO2 |
| S1S2 of AtGLR3.3 | (Alfieri et al., 2020) | PDB: 6R88 |
| Ligand-binding domain of NMDA receptor | (Jalali-Yazdi et al., 2018) | PDB ID: 6MMS |
| Experimental Models: Cell Lines | ||
| COS-7 | ATTC | CRL-1651 |
| E. coli Origami B (DE3) | Novagen | Cat# 70837 |
| Recombinant DNA | ||
| pEF1-YC3.6 | Dr. Jörg Kudla lab, Univ. Muenster, Germany | N/A |
| Oligonucleotides | ||
| AtGLR3.2 amplification primer: 5’-gtaacggccgccagtgtgctggaattcA TGTTTTGGGTTTTGGTTCTGT-3’ | This paper | N/A |
| AtGLR3.2 amplification primer: 5’- atagggccctctagatgcatgctcgaGTCATATTGGTCTAGAAGGT-3’ | This paper | N/A |
| pcDNA3 | Invitrogen | N/A |
| pCI-AtCNIH4 | Wudick et al., 2018b | Genebank: NC_003070.9; At1g12390; Salk_145991 |
| pcDNA3-AtGLR3.2 | This paper | GeneBank: NC_003075; Araprot: At4G35290 |
| pET22b-GLR3.2-S1S2 | This paper | GeneBank: NC_003075; Araprot: At4G35290 |
| Software and Algorithms | ||
| Pymol (Schrödinger) | DeLano, 2002 | http://www.pymol.org |
| PHENIX | Adams et al., 2010 | https://www.phenix-online.org/ |
| CCP4 | Winn et al., 2011 | http://www.ccp4.ac.uk/ |
| COOT | Emsley et al., 2004 | http://www2.mrc-lmb.cam.ac.uk/Personal/pemsley/coot |
| XDS | Kabsch, 2010 | http://xds.mpimf-heidelberg.mpg.de/ |
| Swiss-Model | Waterhouse et al., 2018 | https://swissmodel.expasy.org/ |
| PDBsum | Laskowski et al., 2018 | https://www.ebi.ac.uk/thornton-srv/databases/cgi-bin/pdbsum/GetPage.pl?pdbcode=index.html |
| SigmaPlot 11.0 | Systat Software Inc. | Systatsoftware.com |
| Other | ||
| DeltaVision Elite Deconvolution/TIRF microscope system | GE Healthcare | Part # 53–851206-001 |
| Ion Exchange Hi-Trap Q HP column | GE Healthcare | Cat# 17–1154-01 |
| Size Exclusion Superose 10/300 column | GE Healthcare | Cat# 17–5172-01 |