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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2020 Dec 8;20(2):274–283. doi: 10.1158/1535-7163.MCT-20-0567

Figure 2. Leflunomide induced G1 cell cycle arrest and attenuated the growth of H460 xenograft.

Figure 2.

(A) SRB analysis of leflunomide dose-response in H460 cells from Day 1 to 3. (B) H460 cells were treated with 1 mM AICAR, 0 mM, or 100 μM leflunomide for 48 hrs. Cell lysates were analyzed by immunoblot using indicated antibodies. (C) Cell cycle analysis of H460 cells treated with 0 or 100 μM leflunomide for 48 hrs. (D) H460 xenograft growth over the 23-day treatment period (X-axis). Y-axis represents Ln(Tumor Volume in mm3). (E). Tumor Weight (g) comparison in the control and treated group with indicated Kruskal-Wallis p-value. (F) Photos of isolated H460-xenografts at the end of the study. Four xenografts on the left were harvested on Day 21, and the remaining xenografts were collected on Day 23.