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. Author manuscript; available in PMC: 2022 Feb 4.
Published in final edited form as: Cell Stem Cell. 2020 Nov 10;28(2):230–240.e6. doi: 10.1016/j.stem.2020.10.013

Figure 5. Embryonic Organoids Recapitulate Cardiac Morphogenesis.

Figure 5.

(A) Light-sheet imaging of cleared gastruloids from 144 to 168 h show an initial crescent-like domain that is condensed into a beating bud at 168 h.

(B) Schematic illustrating the comparison between gastruloid stages of cardiac development and embryonic stages from cardiac crescent to linear heart tube.

(C) Quantification of geometrical properties (spareness) of the gastruloid cardiac domains compared to those of defined artificial shapes, with n = 31 gastruloids at 144 h and n = 27 gastruloids at 168 h.

(D and E) The cardiac domain is localized near the anterior epithelial gut-tube-like structure, separated by a CD31+ endocardial layer.

Scale bars, 100 μm. See also Figure S4.