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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Mater Sci Eng C Mater Biol Appl. 2020 Nov 27;120:111748. doi: 10.1016/j.msec.2020.111748

Figure 7.

Figure 7

(I) Recombinant BMP-9 induced ectopic bone formation using the sonoporation or electroporation method. Micro-CT images in high resolution for the detection of ectopic bone formation in thigh muscles of the mouse model using (a) sonoporation and (b) electroporation; 3D representation of the ectopic bone formation induced in vivo. 2D image of representative ectopic bone formation showed an enlarged format on the left of (a). Ectopic bone formation quantitative analysis of bone volume (c), volume density of the formed bone (d), and mineral density of the ectopic bone segment (e). Standard error bars shown for n=9. (II) Histology evaluation of the ectopic bone formation induced by rhBMP-9 in the in vivo mouse model. After 6 weeks post-sonoporation (a and b) H&E staining results and (c and d) Masson trichrome staining data; BF – bone formation, BM – bone marrow, M – skeletal muscle; osteocytes are identified with the yellow arrows. Figure 7. (I) and (II) - Reprinted by permission from Springer Nature: Gene Therapy [144], D. Sheyn, N. Kimelman-Bleich, G. Pelled, Y. Zilberman, D. Gazit, Z. Gazit, Ultrasound-based nonviral gene delivery induces bone formation in vivo. Copyright © 2007, Springer Nature.