Transplantation of aged male fecal microbiota causes systemic inflammation and metabolic disorders. The expression of inflammation and metabolism related genes and protein in the liver (A,B), adipose tissue (C), and ileum (D) in mice of both sexes with and without aged male fecal microbiota transplantation (AFMT). n=4 per group. Data are expressed as mean ± SD. One-way ANOVA with Tukey’s correction. *, P<0.05; **, P<0.01; ***, P<0.001. Tnfα, tumor necrosis factor alpha; Il6, interleukin 6; Il1β, interleukin 1 beta; Saa1, serum amyloid A1; Icam1, intercellular adhesion molecule 1; Pepck, phosphoenolpyruvate carboxykinase; G6pase, glucose-6-phosphatase; Ifnγ, interferon gamma; Tgr5, Takeda G protein receptor 5.