Skip to main content
. 2021 Jan;10(1):57–70. doi: 10.21037/tlcr-20-1062

Figure 5.

Figure 5

MiR-140-3p could abate E2F7 suppression in LUAD cells. (A) Venn plot showing the overlap of predicted target genes of miR-140-3p by RNA22, PicTar, Mirmap, MircoT, and Targetscan databases. (B) The levels of ANGEL1, TRPS1 and E2F7 in A549 and H1299 cells transfected with miR-NC or miR-140-3p were detected by qRT-PCR. (C) The binding sequences between miR-140-3p and E2F7-3’UTR was shown. The red sequences represent its mutant. (D) The luciferase activity of E2F7-wt and E2F7-mut in A549 and H1299 cells was detected by dual-luciferase reporter assay after transfection of miR-140-3p or miR-NC. (E) The E2F7 expression in 59 pairs of LUAD tissues and adjacent normal tissues was tested. (F) The effect of miR-140-3p on the expression level of E2F7 protein in A549 and H1299 cells was detected by Western blot. (G) The changes of E2F7 protein level in LUAD cells transfected with circ-AASDH or circ-AASDH + miR-140-3p. (H,I) The correlation between E2F7 and circ-AASDH or miR-140-3p was tested. *P<0.05, **P<0.01, ***P<0.001.