Table 3. Sensitivity, specificity, and Youden index of single parameters according to optimum cut-off values in the iron deficiency anemia (IDA), β-thalassemia trait (β-TT), and α-thalassemia trait (α-TT) groups.
| Indices | Groups | AUC (95% CI) | Optimal cut-off | Sensitivity (%) | Specificity (%) | +LR | −LR | Youden index (%) |
|---|---|---|---|---|---|---|---|---|
| RBC | IDA/α-TT | 0.651 (0.575–0.726) | 4.51 | 56.1 | 68.2 | 1.76 | 0.64 | 24.3 |
| IDA/β-TT | 0.680 (0.593–0.766) | 4.66 | 56.25 | 77.35 | 2.48 | 0.57 | 33.60 | |
| HGB | IDA/α-TT | 0.610 (0.522–0.699) | 108 | 30.30 | 95.29 | 6.44 | 0.73 | 25.76 |
| IDA/β-TT | 0.510 (0.415–0.605) | 106 | 20.83 | 89.41 | 1.97 | 0.89 | 10.25 | |
| HCT | IDA/α-TT | 0.612 (0.525–0.700) | 35 | 28.79 | 97.65 | 12.23 | 0.73 | 26.43 |
| IDA/β-TT | 0.519 (0.428–0.610) | 33.7 | 22.92 | 84.71 | 1.50 | 0.91 | 7.62 | |
| MCV | IDA/α-TT | 0.606 (0.540–0.672) | 73.8 | 83.33 | 43.53 | 1.48 | 0.38 | 26.86 |
| IDA/β-TT | 0.715 (0.649–0.780) | 69.3 | 64.58 | 74.12 | 2.50 | 0.48 | 38.70 | |
| MCH | IDA/α-TT | 0.591 (0.522–0.660) | 23.5 | 90.91 | 32.06 | 1.34 | 0.28 | 22.97 |
| IDA/β-TT | 0.714 (0.650–0.779) | 21.9 | 75.0 | 65.0 | 2.14 | 0.38 | 40.0 | |
| RDW-CV | IDA/α-TT | 0.536 (0.456–0.616) | 14.7 | 62.12 | 49.71 | 1.24 | 0.76 | 11.83 |
| IDA/β-TT | 0.638 (0.573–0.702) | 14.7 | 81.25 | 50.29 | 1.63 | 0.37 | 31.54 | |
| Ret# | IDA/α-TT | 0.583 (0.498–0.668) | 0.116 | 33.33 | 88.24 | 2.83 | 0.76 | 21.57 |
| IDA/β-TT | 0.781 (0.704–0.859) | 0.108 | 70.83 | 77.94 | 3.21 | 0.37 | 48.77 | |
| Ret% | IDA/α-TT | 0.507 (0.420–0.595) | 3.34 | 15.15 | 97.94 | 7.36 | 0.87 | 13.09 |
| IDA/β-TT | 0.693 (0.605–0.781) | 2.7 | 45.83 | 87.06 | 3.54 | 0.62 | 32.89 | |
| RPI | IDA/α-TT | 0.602 (0.518–0.687) | 1 | 45.45 | 74.12 | 1.76 | 0.74 | 19.57 |
| IDA/β-TT | 0.711 (0.628–0.794) | 0.9 | 68.75 | 62.94 | 1.86 | 0.50 | 31.69 | |
| %MIRCOr | IDA/α-TT | 0.528 (0.460–0.596) | 1.02 | 77.27 | 35.88 | 1.18 | 0.68 | 13.16 |
| IDA/β-TT | 0.587 (0.509–0.666) | 1.64 | 85.42 | 34.71 | 1.31 | 0.42 | 20.12 | |
| MCVm | IDA/α-TT | 0.573 (0.503–0.643) | 68.51 | 84.85 | 35 | 1.31 | 0.43 | 19.85 |
| IDA/β-TT | 0.686 (0.620–0.751) | 66.26 | 91.67 | 44.71 | 1.66 | 0.19 | 36.37 | |
| MHA 1 | IDA/α-TT | 0.868 (0.822–0.914) | 0.61 | 90.91 | 68.63 | 2.89 | 0.13 | 59.44 |
| IDA/β-TT | 0.518 (0.738–0.874) | 4.39 | 27.08 | 79.71 | 1.33 | 0.91 | 48.92 | |
| MHA 2 | IDA/α-TT | 0.772 (0.712–0.833) | 0.85 | 78.79 | 64.12 | 2.20 | 0.33 | 42.91 |
| IDA/β-TT | 0.878 (0.824–0.932) | 0.37 | 72.92 | 89.71 | 7.08 | 0.30 | 62.62 |
RBC, red blood cell; HGB, hemoglobin concentration; HCT, hematocrit; HGB, hemoglobin; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; RDW-CV, red blood cell distribution width coefficient of variation; RDW-SD, red blood cell width standard deviation; Ret#, reticulocyte number; Ret%, reticulocyte percentage; RPI, reticulocyte production index; %MICROr, the proportion of reticulocytes less than 60 fL in volume; MCVm, mean corpuscular volume of mature red blood cell; MHA 1, formula for distinguishing α-TT carriers from IDA cases; MHA 2, formula for distinguishing β-TT carriers from IDA cases.