Figure 4.

Nude β-CD-GSH-NSs are not toxic for normal fibroblasts. Fibroblasts were plated on sterile coverslips and treated for 72 hours with C6-GSH-NSs administered in single dose with no change of the medium (A) or in three doses (every 24 h) by renovating the treatment (B). Cells were labeled with Cell Tracker Blue fluorescent dye (left panel) or with Propidium iodide (PI) (right panel). Coverslips were washed and mounted on glasses and imaged immediately at the fluorescent microscope. Graphs represent the quantification of fluorescence intensity (INT.DEN. average per cell ± SD) for Cell Tracker staining, while cell death was assessed by counting the percentage of PI positive cells and represented in the graphs (% ± SD). Representative images of three independent experiments are shown.