Table 3. Characteristics of 14 microsatellite primers developed for Seseli farrenyi.

Locus	Primer sequence (5′→3′)	Ta (°C)	Repeat motif	Size range (bp)	N	GenBank accession no.
SFA21	F: *GCG GGA ATA AGG AGG AGA GT R: ATT CGG GCA CCT TAA ATC CT	59	(AG)13	154–178	100	MT628686
SFA9	F: *CAA AAC AGG GGG TTA TCA TTG R: TTT GGG GAT CTT GTC CAT CT	59	(GA)9	192	7	MT628687
SFA121	F: TTT GAA TTG AAT AGG TTG CAA GAA R: *TCT CGA CAG CTC AAC CGT AA	60	(GT)13(GA)16	194–236	100	MT628688
SFA151	F: *ACC TCG GTA TCA TCA ACC TG R: ATC AAG GCC TTT CGA ATA CC	58	(GT)17	179–209	100	MT628689
SFA181	F: *AGT AGC AAG GTC CGC GTA AA R: GGA AGC TGA GCT ATG CTG ATG	60	(CT)10	149–169	100	MT628690
SFA241	F: *CTT CGA CAC TGC ACC AAA GA R: GAC ATC TTA ACG GTC CAA GAG C	60	(CT)14(CA)26	246–274	100	MT628691
SFA311	F: *AGA GCT TTT GGG TTT GCG TA R: AAG ACC CCT TTC CAG CTT GT	60	(CT)12	216–224	100	MT628692
SFA321	F: CCA ACA AAT CAA TTA ATC ACA CC R: *TGG GTC GAA TAT TAT GGA TGG	59	(AG)32	185–211	100	MT628693
SFA351	F: *CGC AAG CAC AGA GGG ATA AT R: TGG GGG TAA TTG GTG CTC TA	60	(GT)17	186–204	100	MT628694
SFA46	F: TCC CAT TTG TTA GAA CCT GTT G R: *ACA ATA CTT GGG TCT CTG TCG	56	(AG)23	228	7	MT628695
SFA47	F: *GCT ATG CGC CCG TAG TTT AC R: ACG CGT GGA CTA ACA TCT CTC	59	(GA)30	193	7	MT628696
SFA481	F: *AAT AAA CTG GCG TGC AAT CA R: CAC TGG CAA TCC ATT ATG CTA A	59	(GT)10(GA)16	153–181	100	MT628697
SFA55	F: GTA CAT CAA GAT AAA TAT ACA CAA ACA R: *CGC CCT TGT AGT TTT CAT TA	55	(GA)28	212	7	MT628698
SFA57	F: *GCC CTT TCA ACA ATC ATC CA R: GTG AGT TCG TGG GTT GGT GT	60	(AC)14	149	7	MT628699

Notes:

¹Selected polymorphic loci.

^*M13 tail (CACGACGTTGTAAAACGAC).