Table 1. Existing and newly designed primers used in this study.
| Target gene | Primer | Sequences | Approximate amplicon length (bp) | References |
|---|---|---|---|---|
| PKSI | K1F | TSAAGTCSAACATCGGBCA | 1,200–1,400 | Ayuso-Sacido & Genilloud (2005) |
| M6R | CGCAGGTTSCSGTACCAGTA | |||
| PKSI | KSDPQQF | MGNGARGCNNWNSMNATGGAYCCNCARCANMG | 700 | Ayuso-Sacido & Genilloud (2005) |
| KSHGTGR | GGRTCNCCNARNSWNGTNCCNGTNCCRTG | |||
| PKSI | MDPQQRF | RTRGAYCCNCAGCAICG | 750 | Kim & Fuerst (2006) |
| HGTGTR | VGTNCCNGTGCCRTG | |||
| PKSII | PF6 | TSGCSTGCTTGGAYGCSATC | 600 | Metsa-Ketela et al. (1999) |
| PR6 | TGGAANCCGCCGAABCCGCT | |||
| NRPS | MTF | CCNCGDATYTTNACYTG | 750–1,000 | Tambadou et al. (2014) |
| MTR | GCNGGYGGYGCNTAYGTNCC | |||
| NRPS | A3F | GCSTACSYSATSTACACSTCSGG | 700 | Ayuso-Sacido & Genilloud (2005) |
| A7R | SASGTCVCCSGTSCGGTAS | |||
| 16S rRNA | 27f | AGAGTTTGATCMTGGCTCAG | 1,400–1,600 | Chen et al. (2015) |
| 1492r | ACGGYTACCTTGTTACGACTT | |||
| 16S rRNA | 331f | TTCTACGGGAGGCAGCAGT | 460 | Chan et al. (2018) |
| 797r | GGACTACCAGGGTATCTAATCCTGTT | |||
| COI | LCO1490-JJ | GGTCAACAAATCATAAAGATATTGG | 708 | Astrin & Stüben (2008) |
| HCO2198-JJ | TAAACTTCAGGGTGACCAAAAAATCA | |||
| Unidentified bacteria | 1F | TACATAGGGTGCGAGCG | 220 | This study |
| 220R | GTCAGTACATTCCCAGTTAGT | |||
| 207R | CAGTTAGTTGCCTTCGCCATT | |||
| Gamma-proteobacteria (A) | 44F | GTGCGTGGCGGCATGAT | 145 | This study |
| 179R | TCCCGATATCTACGCATTC | |||
| Gamma-proteobacteria (B) | 41F | AGCGCACGTAGGTGGTGCGG | 180 | This study |
| 222R | TGTCAGTTACAGTCCAGGTGT | |||
| 56F | TGCGGTAAGCCAGATGTGAAA | 180 | This study | |
| 233R | TCGCTCCTCAGTGTCAGTTA |