Table 4. Isolate identification, presence of natural product gene fragments (polyketide synthase types PKS I, PKS II and non-ribosomal peptide synthetase NRPS), and bioactivity of crude extracts in antimicrobial and antitumor assays.

Nudibranch	Strain	Closest 16SrRNA gene match	Accession no.	Identity (%)	PCR screening			Bioactivity*
					PKS I	PKS II	NRPS	Anti- bacterial	Anti- tumor
Joruna funebris	1AS	Nocardiopsis sp. AE46	JF319150	99.91	+	–	+	++	+++
	2AS	Nocardiopsis dassonvillei subsp. albirubida VTT E-062983	EU430536	100.0	+	+	+	+++	++
	8AS	Bacillus safensis strain HA 527	KJ535336	99.8	–	+	+	+	–
	18AS	Bacillus sp. strain HBS1	MK966451	99.9	–	+	+	++	++
	24AS	Bacillus flexus strain CPS1.1	MT299668	99.85	–	+	–	+	+
	25AS	Staphylococcus lentus strain HS1-MRL	KX128918	100.0	+	+	–	+	+
	16A	Bacillus aerius strain FL101	KY819007	99.4	+	–	+
Ceratosoma trilobatum	2BY	Streptomyces xylophagus strain TY190-20	MT083969	100.0	–	+	+	+	–
	4BY	Bacillus sp. S21722	KF956683	99.8	–	+	+	–	–
	6By	Bacillus safensis strain P9	MK210556	99.42	+	+	+	–	–
	11By	Bacillus paramycoides strain MMB	MT122838	99.88	–	+	+	+	–
	12BY	Bacillus sp. 6063	JX566648	99.9	+	+	+	++	–
	13BY	Nocardiopsis dassonvillei subsp. albirubida strain OAct926	MG661750	98.8	+	+	+	+++	+++
	15BY	Bacillus sp. S4713	JQ819877	99.9	–	+	+	++	+
	16BY	Kocuria sp. strain YKFH1121	MH298696	99.8	+	+	+	+	–
	23M1	Nocardiopsis dassonvillei strain XY236	MH432693	100	+	+	+	++	+++
Chromodoris quadricolor	6	Bacillus safensis strain SH10	MT256302	99.9	–	+	+	++	–
	9	Bacillus sp. strain C2-8	MT255139	100	+	+	+	+	–
	12	Bacillus altitudinis strain EB39	MT256105	100	+	–	+	+	+++
	14	Oceanobacillus iheyensis strain S6	MN056009	99.7	+	–	+	+	–
	16	Bacillus australimaris strain 96J27	MT192471	100	+	–	+	++	–
	17	Bacillus safensis strain NS3	KP279980	99.64	–	+	+	+++	++
	20	Staphylococcus lentus strain Hanna37	MN399938	99.64	+	–	+	+	–
	22	Pseudomonas stutzeri strain OsEnb_ALM_B7	MN889324	10	+	+	+	+	–
	24	Planococcus ruber strain CD8	MK216760	99.83	+	+	+	++	–
	31	Bacillus amyloliquefaciens strain VBS03	MG660863	99.85	–	+	+	–	+
	34	Streptomyces sp. E5N406	CP029624	99.7	+	+	+	++	+++
	38	Staphylococcus lentus strain PL445	MK015783	99.93	+	–	–	+	–
	41	Enterococcus sp. M190262	CP040461	99.93	+	+	+	–	–
	43	Rhodococcus erythropolis strain I-A-R-27	KT922050	99.3	+	+	+	+	–
	47	Bacillus sp. strain 22DM7	MK134623	99.8	+	+	+	–	–
	48	Oceanobacillus kimchii MXR1709B02	MN176502	99.8	+	+	–	–	–
	49	Bacillus sp. strain 1RM6 - 0	MK134620	99.8	+	–	+	–	–
	53	Firmicutes bacterium 00YDJ	EU180852	99.8	+	–	+	+++	–
	57	Bacillus velezensis strain 9D-6	CP020805	99.4	+	+	+	+++	++
	59	Streptomyces qinglanensis strain A5	MF682454	99.6	+	+	+	+++	–
	63	Streptomyces sp. SCSIO 001680	JQ031555	100	+	+	+	+++	+
	66	Streptomyces sp. B24AT	KU382279	100.0	+	+	+	+	–
	80	Bacillus pumilus strain B6	KJ870186	99.45	+	+	+	+	–
	2R	Nocardiopsis sp. I-Gauze-W-10-6	FJ267558	99.85	+	+	+
	4R	Streptomyces sp. Ahbb4	KM214828	99.6	+	+	+
	7R	Bacterium strain BH8S5	MT254881	99.93	–	+	+
	8R	Actinomycetales bacterium XJSS-18	EU598254	100.0	+	+	+
	2N	Bacillus flexus strain S72	MN005931	100.0	–	+	+
	10N	Staphylococcus lentus strain HM17	MN401134	99.7	–	+	+
	1AIA	Oceanobacillus iheyensis strain CN6-12	MH762168	99.6	+	+	+
	4AIA	Staphylococcus sp. strain BH1-3	MN410649	100	+	+	+
	3ISP2	Bacillus siamensis strain ICMP 20282	MF682396	99.7	+	+	+
	6M1	Bacillus safensis strain SPa22LB	MT052639	100.0	+	+	+
	10M1	Bacillus sp. strain 9RM71	MK134622	99.7	+	+	+
	14M1	Oceanobacillus iheyensis HTE831	NR075027	99.8	+	+	+
	15M1	Bacillus sp. HNA3	CP040881	100	+	+	+
	16M1	Streptomyces sp. E2N173	KX279582	99.6	+	+	+
	17M1	Bacillus altitudinis strain IHB B1644	MT328683	100.0	+	–	+
	18M1	Streptomyces sp. strain MJM15241	MN636769	100.0	+	+	+
	1S.C	Staphylococcus sp. SC1	KC951997	99.7	+	+	+
	3S.C	Nocardiopsis dassonvillei strain NRK12	MH598413	99.8	–	+	+
	7 S.C	Staphylococcus lentus strain PL444	MK015780	100.0	+	+	–
Goniobranchus annulatus	15(2)	Bacillus sp. strain ZX1	MT305953	100.0	–	+	+
	23(2)	Kocuria sp. strain C12-38	MT255185	100.0	+	+	+
	25(2)	Bacillus zhangzhouensis strain 1.4517	MT316475	100.0	+	+	+
	31(2)	Halobacillus hunanensis strain JSM 071077	LT714153	100.0	–	+	+
	32(2)	Bacillus safensis strain T13	MT313116	100.0	+	–	+
	34(2)	Kocuria carniphila strain NF28	MT262515	99.9	+	+	+
	36(2)	Kocuria carniphila strain YM	JX485387	100.0	+	+	+
	47(2)	Bacillus sp. strain YB-3	MF661927	100.0	–	+	+
	59(2)	Nocardiopsis sp. 06-St-023	GU574127	99.9	+	+	+
	78(2)	Bacillus subtilis strain RI4914	CP051306	100.0	–	+	+

Note:

^*For antimicrobial activity, zone of inhibition: + <10 mm; ++ ≤15 mm; and +++ ≤ 20. For antitumor activity, IC₅₀: measured with µg/mL, + > 700; ++ < 700 µg/mL; +++ < 400 µg/mL. − = no activity.