(A) TRAP staining of cultured bone marrow hematopoietic cells, and (B) quantification of TRAP+ cells. (C–E) Changes in the gene expression of bone resorption markers in cultured bone marrow hematopoietic cells determined by quantitative PCR: (C) TRAP, (D) integrin β3, and (E) calcitonin receptor (CTR). *p<0.05, **p<0.01, ***p<0.001 by one-way ANOVA plus Newman–Keuls post hoc test, n=6–8 per group.