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. 2021 Feb 3;14:751–761. doi: 10.2147/OTT.S240010

Figure 2.

Figure 2

MCTP1-AS1 inhibits cell proliferation, cell migration, cell invasion and EMT process in EC cells. (A) The transfection efficiency of Ishikawa cells in response to overexpression MCTP1-AS1 plasmid, as detected by quantitative RT-PCR (**p<0.01). (B) Cell proliferation was measured in Ishikawa cells overexpressed MCTP1-AS1 plasmid using CCK8 at 0 h, 24 h, 48 h and 72 h time points. Data are mean ± SD of triplicate experiment (**p<0.01). (C) Wound healing assay was performed in both cell lines following transfection with MCTP-AS1 overexpression plasmid. (D) Transwell assay was used to measure the effect of MCTP1-AS1 overexpression on cell migration and invasion in Ishikawa cells. Data are mean ± SD of triplicate experiment (**p<0.01). (E) MCTP1-AS1 overexpression significantly promotes the expression of E-cadherin but inhibited N-cadherin and vimentin.