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. 2021 Feb 3;14:751–761. doi: 10.2147/OTT.S240010

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© 2021 Gao et al.

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MCTP1-AS1 regulated EMT process via miR-650 in EC cells. (A) miR-650 mimic was transfected into Ishikawa cells, and the transfection efficiency was verified using qRT-PCR. Data are mean ± SD of triplicate experiment (**p<0.01). (B) Cell proliferation was measured in MCTP1-AS1 overexpressed Ishikawa cells transfected with miR-650 mimic using CCK8 at 0 h, 24 h, 48 h and 72 h time points. Data are mean ± SD of triplicate experiment (**p<0.01). (C) Wound healing assay was performed in MCTP1-AS1 overexpressed Ishikawa cell lines following transfection with miR-650 mimic. (D) Transwell assay was used to measure the effect of miR-650 overexpression on cell migration and invasion in MCTP1-AS1 overexpressed Ishikawa cells. Data are mean ± SD of triplicate experiment (**p<0.01). (E) Western blot shows miR-650 overexpression significantly reverses the effect of MCTP1-AS1 on E-cadherin, N-cadherin and Vimentin expression in EC cells.