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. 2021 Feb 8;38(2):21. doi: 10.1007/s12032-021-01472-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — NAC reverses the inhibitory effects of pyrvinium. a For Western blot analysis, HCT116 cells were treated with increasing concentrations of pyrvinium for 24 h. Cells were treated with pyrvinium (0.50 μM) in the absence and presence of NAC (5 mM), and then intracellular ROS (b), cell viability (c), and the expression of AKT and Ki67 in HCT116 cells (d) were measured. **P < 0.01, ***P < 0.001