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. 2021 Jan 25;11:608294. doi: 10.3389/fimmu.2020.608294

Figure 2.

Figure 2

Lack of RGC-32 results in a higher proliferative index in spinal cord astrocytes during acute EAE. Spinal cords from WT (A, C) and RGC-32 KO mice (B, D) were harvested at the peak of EAE (day 14) and double-stained with anti-Ki-67 (red) and anti-GFAP (blue) antibody. In WT mice, Ki-67-positive astrocytes were mainly present around the inflammation (A, arrows) with few cells outside inflammatory infiltrate (C, arrow). In contrast, in RGC-32 KO mice, we noticed abundant Ki-67-positive astrocytes throughout the spinal cords (B, D, arrows). Original magnification: (A, B): x20; (C, D): x40. Scale bars (A, B): 40 µm; (C, D): 20 µm. Ki-67-positive/GFAP-positive and Ki-67-positive/GFAP-negative cells were manually counted in white matter areas from 10x-magnified spinal cord sections, and the values were compared for WT and RGC-32 KO spinal cords. We found a higher number of Ki-67/GFAP double-positive cells in the RGC-32 KO mice (E) and a lower number of Ki-67-positive/GFAP-negative cells in these mice than in the WT mice (F) because of the presence of inflammatory cells positive for Ki-67 (A, arrowheads). Results in (E, F) are expressed as mean ± SEM (n = 7 areas per 3 mice in WT: n = 6 areas per 3 mice in RGC-32 KO). ** = p<0.01; *** = p<0.001.