Table 1.
Real Time PCR array.
| Gene name | Genbank accession | WT fold change | KO fold change | WT/KO ratio |
|---|---|---|---|---|
| Insulin-like growth factor 1 receptor (IGF1R) | NT_187035.1 | 52.35 | 2.46 | 21.28 |
| Collagen type I alpha 1 (COL1A1) |
NT_096135.6 | 60.02 | 4.33 | 13.86 |
| Ezrin (EZR) | NT_039636.8 | 36.78 | 2.78 | 13.23 |
| Insulin-like growth factor 1 (IGF1) |
NT_039500.8 | 17.88 | 3.57 | 5 |
| Matrix metallopeptidase 13 (MMP13) |
NT_039471.8 | 0.31 | 0.09 | 3.4 |
| Epidermal growth factor receptor (EGFR) | NT_039515.7 | 2.84 | 0.98 | 2.9 |
| CD44 antigen | NT_039207.8 | 5.87 | 2.26 | 2.6 |
| Matrix metallopeptidase 3 (MMP3) |
NT_039471.8 | 1.49 | 0.67 | 2.22 |
| Kallikrein 1-related peptidase b16 (KLK1B16) | NT_187035.1 | 2.38 | 1.17 | 2.03 |
| Serine (or cysteine) peptidase inhibitor, clade E, member 1 (SERPINE1) | NT_039314.8 | 12.43 | 18.62 | 0.66 |
| Matrix metallopeptidase 14 (MMP14) |
NT_039606.8 | 1.72 | 2.79 | 0.61 |
| Tissue inhibitor of metalloproteinase 1 (TIMP1) | NT_039700.8 | 3.75 | 6.89 | 0.54 |
| Plasminogen activator, urokinase receptor (PLAUR) | NT_187034.1 | 0.81 | 2.92 | 0.27 |
| Matrix metallopeptidase 9 (MMP9) |
NT_039207.8 | 0.44 | 1.69 | 0.26 |
WT and RGC-32 KO mouse astrocytes were stimulated with TGF-β1 (10 ng/ml) for 24 hours and total RNA was purified, reverse transcribed and subjected to a PrimePCR ECM remodeling array, according to manufacturer’s instructions. Each transcript was normalized to GAPDH. Quantification was performed by using the ΔΔCT method of relative quantification. The values are shown as fold change over unstimulated, which was considered 1. The results show the ratios with at least 1.5-fold difference between WT and KO.