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. 2021 Jan 25;13:610037. doi: 10.3389/fnmol.2020.610037

Figure 1.

Figure 1

Schematic of recombinant tau proteins used. (A) hTau40-WT corresponds to the longest isoform of human CNS tau containing two N-terminal inserts (exons 2 and 3) and four microtubule-binding repeats. (B) The hTau40-S199E, hTau40-S202E, and hTau40-T205E recombinant proteins were each pseudophosphorylated by a single point mutation (S$→$E or T$→$E) of the AT8 epitope. (C) The hTau40-S199E/S202E and hTau40-S202E/T205E recombinant proteins were pseudophosphorylated at two combinations of the individual mutation sites. (D) Each recombinant tau protein was run on SDS–PAGE (1 μg/lane) and the gel was stained with Coomassie to show each protein. DnaK (~70 kDa) copurifies with tau protein (Combs et al., 2017).