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. 2021 Jan 25;12:644013. doi: 10.3389/fmicb.2021.644013

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Copyright © 2021 Kumar, Meghvansi and Kamboj.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology, adsorption rate, and one-step growth curve of bacteriophage HCF1. (A) Bacteriophage plaque in double agar plating assay. (B) Transmission electron micrograph. (C) The rate of adsorption of bacteriophage to the C. amalonaticus NAIMCC 1364 host. Bacteriophages were added to the bacterial suspension at an MOI:0.1. The percentage of non-adsorbed bacteriophages was calculated at the indicated time points. The presented data are means of three independent experiments with error bars showing the standard error of mean (SEM). (D) One-step growth curve analysis of bacteriophage HCF1. Plaque-forming units (PFU) were estimated at different intervals post infection against host bacterium C. amalonaticus. Bars represent SEM.