TABLE 2.

In vitro assay of bacteriophage HCF1 against Citrobacter amalonaticus in saline solution and in an anaerobic biodigester effluent.

Conditions	Time	Control	MOI 1	MOI 10	MOI 100
Saline	0h	8.80 ± 0.04a	8.46 ± 0.09a	8.64 ± 0.07a	8.54 ± 0.27a
	1h	8.76 ± 0.04a	7.53 ± 0.12b	7.65 ± 0.09b	6.53 ± 0.07c
	2h	8.72 ± 0.05a	6.48 ± 0.09b	5.35 ± 0.19c	4.52 ± 0.14d
	3h	8.63 ± 0.06a	5.28 ± 0.20b	4.28 ± 0.20c	2.51 ± 0.13d
	4h	8.55 ± 0.08a	4.59 ± 0.06b	3.45 ± 0.23c	0.00*d
	5h	8.44 ± 0.07a	3.67 ± 0.04b	0.00*c	0.00*c
	6h	8.24 ± 0.16a	2.19 ± 0.12b	0.00*c	0.00*c
	24h	7.53 ± 0.12a	0.00*b	0.00*b	0.00*b
Anaerobic biodigester effluent	0h	8.89 ± 0.06a	8.74 ± 0.14a	8.42 ± 0.25a	8.67 ± 0.11a
	1h	8.81 ± 0.11a	7.57 ± 0.09b	7.67 ± 0.11b	6.51 ± 0.17c
	2h	8.74 ± 0.07a	6.66 ± 0.07b	5.65 ± 0.09c	3.65 ± 0.12d
	3h	8.68 ± 0.10a	4.57 ± 0.14b	3.65 ± 0.03c	2.42 ± 0.21d
	4h	8.61 ± 0.22a	3.62 ± 0.09b	2.73 ± 0.10c	0.00*d
	5h	8.67 ± 0.04a	2.79 ± 0.10b	0.00*c	0.00*c
	6h	8.26 ± 0.18a	2.23 ± 0.12b	0.00*c	0.00*c
	24h	9.46 ± 0.14a	0.00*b	0.00*b	0.00*b

*No growth was observed; log-transformed CFU values are presented in the table. ± Standard error of mean (n = 3). Values of a given bacteriophage depicted in column without common letter differ significantly at p ≤ 0.05 as per Tukey’s HSD test.