Figure 5.

Proteomic validation of FASN and potential signal pathway (especially the EMT process) in vitro. (A) IHC staining was performed to validate differential expression levels between tumor and normal samples, suggesting a significantly elevated FASN staining density and intensity in ccRCC tissues compared with normal tissues. (B) GSEA also suggested that FASN, together with related hub genes, was significantly involved in epithelial–mesenchymal transition process (NES = 2.073), consistent with the role of FASN-mediated ccRCC cells migration ability. (C) RT-qPCR reveals differential mRNA expression of hub genes in epithelial–mesenchymal transformation process, including cyclin E, cyclin D, E-cadherin, vimentin, and N-cadherin. The finding indicated that inhibition of FASN significantly decreased cyclin E, cyclin D, vimentin, and N-cadherin expression (p < 0.05) and increased E-cadherin mRNA expression (p < 0.01). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.