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. 2021 Jan 25;11:586126. doi: 10.3389/fimmu.2020.586126

Figure 3.

Figure 3

Pro-inflammatory activities of pTA-NKs from PCa patients on endothelial cells. Conditioned media (CM) form FACS-sorted PCa pTA-NKs are enriched in pro-inflammatory and tissue-remodelling factors, such CXCL8, uPAR, MMP-1, MMP-9 (A) and functionally support the formation of capillary like structures in human umbilical-vein endothelial cells (HUVEC) on matrigel (B). HUVE cells exposed to conditioned media of PCa pTA-NKs express higher levels of pro-inflammatory factors like VEGF, VEGF-R2, CXCL8, CXCR4, CXCL12, ICAM-1, VCAM-1, IL1-α, as compared to those exposed to conditioned media released by healthy control NK cells (C). Capillary like-structure formation and qPCR on HUVECs have been performed using CM of pNK cell from 3 PCa patients and 3 controls, in triplicate, on 4 different HC. Data are showed as 2^-ΔCt values, mean ± SEM, ANOVA, *p<0.05, **p<0.01, ****p<0.0001. The condition HUVEC (black bar) stands for HUVE cells alone, as baseline condition. HC, healthy controls; ADK, prostate adenocarcinoma.