Skip to main content
. 2021 Jan 25;11:586126. doi: 10.3389/fimmu.2020.586126

Figure 6.

Figure 6

Effects of prostate cancer cell line conditioned media (CM) on pNK cell polarization and functions. NK cells from healthy donors, following 72 h of exposure to conditioned media (CM) from PC-3, DU-145 and LNCaP PCa cell lines, exhibit a pro-inflammatory angiogenic decidual-like phenotype, as revealed by the increased levels of the dNK-like markers CD9, CD49a, CXCR4 (A, B), enhanced production of pro-inflammatory factors (angiogenin, ANG; angiopoietin-1, Angiop1; CXCL8) and reduced production of cytolytic factors (granzyme B, GRZ-B; TNFα; IFNγ) (C, D), as revealed by flow cytometry analysis. Experiments were performed using peripheral blood samples of 5-to-9 independent healthy donors. NK cells from healthy donors, following 72 h of exposure to conditioned media (CM) from PC-3, DU-145 cell lines increase the expression of PD-1 and TIM-3 exhaustion markers (E), together with decreased degranulation capabilities against K562 cells (F). Experiments were performed using peripheral blood samples of 3 independent healthy donors. Data are shown as mean ± SEM, ANOVA, *p<0.05, **p<0.01, ***p<0.001. CM, conditioned media/conditioned media from 72 h of SFM PCa cell lines.