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. 2021 Jan 25;11:597638. doi: 10.3389/fphys.2020.597638

Figure 3.

Figure 3

(A) Hematoxylin-eosin (HE) stained images in control group, 0, 3, 5, 10, and 14 days after frostbite. Damaged skeletal muscle tissue exhibits diffuse swelling of muscle cells or inflammatory cells infiltration (Horizontal black arrow). On day 10, regenerated skeletal muscle (Vertical black arrow) was observed. On day 14, red blood cells (white arrows) can be observed in muscle tissue. (B) This example shows how to measure the fiber cross-sectional area (fCSA) and extracellular area fraction (EAF). (C,D): EAF and fCSA of control group and frostbite groups. Scale bar, 200 μm. EAF, extracellular matrix area fraction; fCSA, fiber cross-sectional area. ** p < 0.01.