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. 2021 Jan 25;12:606927. doi: 10.3389/fnagi.2020.606927

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Copyright © 2021 Jiang, Ma, Wang, Wang, Yang, Li and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PNU-282987 changes the subsets of CD4+ T lymphocytes and Foxp3 cells in the SN. The immunofluorescence staining of Foxp3/DAPI was performed using antibodies against: CD4 (green stain) and Foxp3 (red stain) and DAPI (blue stain). Merged image showing CD4 or Foxp3 and DAPI staining (n = 6 per group). The scale bar indicates 200 and 20 μm, respectively. (A) 6-OHDA-lesion group; (B) 6-OHDA-lesion + sham group; (C) 6-OHDA-lesion + PNU-282987 group. (D) The graph depicts the positive number of CD4+ cells and Foxp3 cells in the SN from each experimental group. ⁺p < 0.05 compared with the 6-OHDA-lesion group (n = 6 per group), and *p < 0.05 compared with the 6-OHDA-lesion + sham group (n = 6 per group).