Figure 1.

Deletion of HuR does not influence cardiac function under steady-state conditions. (A) PCR and western blot analysis to test the presence of loxP and Cre genes as well as the protein of HuR in the heart tissues from cardiomyocyte-specific HuR-knockout (HuR KO) and wild-type (WT) mice (6–8 weeks old). (B) Representative images of the Doppler imaging of echocardiography. (C) Analysis of diastolic left ventricular anterior wall (LVAW; d), diastolic left ventricular posterior wall (LVPW; d), as well as the ejection fraction (EF) and fractional shortening (FS). Data are the means ± SD from five independent experiments. (D) Heart weight and the ratio between heart and body weight. Data are the means ± SD from five mice. (E) Representative images of H & E staining of the heart and heart slices. (F) WGA staining to evaluate the size of the cardiomyocytes from the heart slices. The relative cells size is shown as the means ± SD from three mice.