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. 2019 Aug 20;116(5):944–955. doi: 10.1093/cvr/cvz205

Figure 3.

Figure 3

The expression of HuR, PLB, β1-AR, and β1-AR alters in response to ISO. (A, Left) C57BL/6J mice were injected with isoproterenol (ISO, 7.5 mg/kg/day). Two weeks later, protein lysates prepared from heart tissues were subjected to western blot analysis to test the protein levels of HuR, PLB, β1-AR, β1-AR, and TUBA. (Right) the intensity of the blots in the left panels is represented as the means ± SD from six mice; significance is analysed by Student’s t-test (**P < 0.01). (B) RNA prepared from the tissues described in (A) was subjected to RT-qPCR to determine the mRNA levels of PLB, β1-AR, and β2-AR. Data are the means ± SD from six mice; significance is analysed by Student’s t-test (*P < 0.05; **P < 0.01).