Figure 5.

HuR ablation alters the expression of PLB and β1-AR in response to ISO treatment. (A, B) Protein lysates prepared from the heart tissues described in Figure 4A were subjected to western blot analysis to determine the protein levels of HuR, PLB, β1-AR, and TUBA (A). The intensity of the blots for PLB and β1-AR was shown as the means ± SD from 6 mice; significance is analysed by Student’s t-test (B) (*P < 0.05). (C) RT-qPCR analysis was used to test the mRNA levels of collagen I (Col I) and collagen III (Col III) in tissues described in (A). Data are the means ± SD from six mice; significance is analysed by Student’s t-test (**P < 0.01).