Skip to main content
. 2019 Aug 20;116(5):944–955. doi: 10.1093/cvr/cvz205

Figure 6.

Figure 6

HuR regulates the mRNA turnover of PLB and β1-AR in cells. (A) RNA pulldown assays were performed by using H9C2 cell lysates and in vitro-transcribed RNAs depicted in Supplementary material online, Figure S3A to test the association of HuR with PLB, β1-AR, and β2-AR mRNAs. p27 5′UTR and CR (coding region) served as positive (P) and negative (N) controls, respectively. A 5-µg aliquot input and binding to TUBA were also assessed. (B) H9C2 cells were transfected with a siHuR for 24 h. Cells were further transfected with each of the pGL3-derived reporters together with a pRL-CMV vector and cultured for an additional 48 h, where upon the relative luciferase activities were determined. Data are the means ± SD from three independent experiments; significance is analysed by Mann–Whitney U test (*P < 0.05; **P < 0.01). (C) H9C2 cells were transfected with a siHuR for 48 h, the half-lives of PLB, β1-AR, and GAPDH mRNAs were evaluated as described in Methods section. The means ± SD from three independent experiments and the statistical significance (Mann–Whitney U test) are indicated (*P < 0.05; **P < 0.01).