Figure 4.

PPARγ acts as an upstream regulator of miR-29b

(A) Schematic illustration of the binding sites of PPARγ in the promoter structures of miR-29b: the position of miR-29b-1: chr6:31221817-31221836 [−]; the position of miR-29b-2: chr1:195018028-195018047 [+]. (B) ChIP-PCR assay showed that PPARγ could bind to the promotor of miR-29b. n = 3 per group. (C) Luciferase assay analysis of the regulation function of PPARγ and miR-29b-1. n = 6 per group. (D) Luciferase assay analysis of the regulation function of PPARγ and miR-29b-2. n = 6 per group. (E) Quantitative real-time PCR analysis of miR-29b expression when C2C12 myotubes were transfected with sh-scramble and PPARγ shRNAs. n = 5, 7, 7. (F) Quantitative real-time PCR analysis of miR-29b expression when C2C12 myotubes were transfected with Fugw and PPARγ overexpression lentivirus. n = 4 per group. (G) Western blot analysis of PPARγ protein level in AngII-induced muscle atrophy in vitro. n = 3 per group. (H) Western blot analysis of PPARγ protein level in AngII-induced muscle atrophy in vivo. n = 3 per group. Data are shown as mean ± SD. ∗p < 0.05. ∗∗p < 0.01. See also Figure S4.