Figure 6.

Overexpression of PPARγ ameliorates AngII-induced muscle atrophy in vitro

(A) Immunofluorescent staining for C2C12 myotubes followed by determination of myotube diameter and quantitative real-time PCR analysis of Atrogin-1 and Murf-1 expression when myotubes were transfected with Fugw and PPARγ overexpression lentivirus in AngII-induced muscle atrophy model. Scale bar: 100 μm. n = 4 for staining and n = 6, 5, 5, 5 for quantitative real-time PCR. (B) Immunofluorescent staining for C2C12 myotubes followed by determination of myotube diameter and quantitative real-time PCR analysis of Atrogin-1 and Murf-1 expression when myotubes were treated with PPARγ agonist rosiglitazone in AngII-induced muscle atrophy model. Ros, rosiglitazone. Scale bar: 100 μm. n = 3 for staining and n = 6 for quantitative real-time PCR. Data are shown as mean ± SD. ∗p < 0.05. ∗∗p < 0.01. See also Figure S6.