Figure 8.

Overexpression of PPARγ ameliorates AngII-induced muscle atrophy in vivo

(A) Quantitative real-time PCR analysis of PPARγ expression in control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. n = 4, 5, 5, 5. (B) Gastrocnemius weight from control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. n = 6. Scale bar is 1cm. (C) The grip strength of right hindlimb from control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. n = 5, 6, 5, 6. (D) WGA staining was performed to quantify muscle fiber CSA from control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. Scale bar: 20 μm. n = 3–4 per group. (E) Quantitative real-time PCR analysis of Atrogin-1 and Murf-1 expressions in control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. n = 5–6 per group. (F) Western blot analysis of Atrogin-1 and MuRF-1 expression in control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. n = 3 per group. (G) TUNEL staining was performed to quantify apoptosis from control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. Scale bar: 20 μm. n = 4 per group. (H) Western blot analysis for the ratio Bax/Bcl2 and Cleaved-caspase 3/Caspase 3 in control and AngII mice treated with Fugw or PPARγ overexpression lentivirus. n = 3 per group. Data are shown as mean ± SD. ∗p < 0.05. ∗∗p < 0.01. See also Figure S8.