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. 2021 Feb 1;11(2):370–388.

Figure 4.

Figure 4

Low concentration of β-elemene induced senescence of glioma cells, but didn’t cause apparent cell apoptosis. (A, B) The effects of 10 μg/mL β-elemene for 1 day or 2 days on C6 cell viability detected by CCK8 (n=3). (C) Western blot detected the expression of and c-caspase-3, caspase-3 and Lamin B1 in cells treated with control or 10 μg/mL β-elemene at for 2 d. (D, E) Quantification of Lamin B1 and c-caspase-3/caspase-3 level as shown in (C) (n=4). (F) Immunostaining analysis of c-caspase-3 (green) in C6 cells treated with control and 10 μg/mL β-elemene for 2 days or with 12 μM etoposide for 1 day, and recovered for 4 days. (G) Quantification of the percentage of c-caspase-3+ C6 cells over total cells as shown in (F) (n=15). d=day. Scale bars, 20 μm. Data were mean ± s.e.m, **P < 0.01, ***P < 0.001.