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. 2021 Feb 1;11(2):416–440.

Figure 6.

Figure 6

YEATS4 mediates EMT in breast cancer cells. (A) mRNA expression changes in EMT markers, including epithelial markers E-cadherin and ZO-1 and mesenchymal markers vimentin and N-cadherin in MDA-MB-231 and SK-BR-3 cells after stable YEATS4 deletion in RT-qPCR assays. (B) Expression changes in EMT markers were evaluated by Western blot analysis in MDA-MB-231 and SK-BR-3 cells after YEATS4 downregulation. (C) Immunofluorescence images in YEATS4 deleting SK-BR-3 and control cells. Scale bar, 20 µm. (D-F) EMT markers in stable T-47D-YEATS4 and ZR-75-1-YEATS4 cells compared with control cells, as determined by RT-qPCR (D), Western blot analysis (E), and immunofluorescence staining (F; scale bar, 20 µm). Data are presented as mean ± s.d. for 3 independent assays in RT-qPCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001.