Figure 3.

Histopathology of hippocampal and cerebral cortex brain tissue at various times after surgery/hypoxia. (A) Brain coronal sections from sham control animals and animals after hypoxia-ischemia were stained with hematoxylin-eosin (H&E). Lower panel represents magnification (200×) of the ipsilateral hemisphere area (marked with rectangles in A). Note the loss of neurons, signs of cerebral edema, and typical red neurons in the cortex of ipsilateral hemisphere at 8 h and 12 h, and these phenomena were slightly alleviated in 24 h. (B) Representative sections of the pyramidal cell layer of the right hippocampus. The HI group (0 h, 4 h, 8 h, 12 h, and 24 h) exhibited the development of features characteristic of acute ischemia, including a decrease in cell density, the appearance of cellular vacuoles, and nuclear pyknosis and fragmentation. Photomicrographs are representative of observations made from eight animals per group.