Expression of NLRP3, caspase-1, and IL-1β proteins in brain tissue evaluated by western blot. A. Representative immunoblots illustrating the expressions levels of NLRP3, caspase-1, and IL-1β proteins in brain tissue from the HI and sham operation groups at varying times (0 h, 4 h, 8 h, 12 h and 24 h) after surgery/hypoxia. B. Averaged semi-quantitative data comparing the expression levels of NLRP3, caspase-1, and IL-1β proteins between the HI and sham operation groups. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham operation group.