Skip to main content
. 2021 Jan 21;41:101873. doi: 10.1016/j.redox.2021.101873

Fig. 2.

Fig. 2

Modulation of HOCl-induced thiol oxidation and GSH/GSSG by SCN−in HCASMC. HCASMC were exposed to 100 μM HOCl with and without addition of SCN− for 15 min. Data in (A) show the total intracellular thiol concentration as determined by the ThioGlo1 assay, immediately following treatment (black bars) or following washing and re-incubation for 24 h (dashed bars) in growth media. Data are normalized to cell protein concentration and expressed as a percentage compared to (ct) the controls. Data in (B), (C) and (D) show the GSH, GSSG and GSH/GSSG ratio, respectively as measured using the GSH/GSSG-Glo™ Assay Kit. GSH and GSSG were measured immediately following the treatments (black bars) and after 24 h re-incubation in cell media (dashed bars) and are expressed as a fold change compared to the non-treated control cells. Statistical significance indicated by *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, ##p < 0.01, ###p < 0.0001 and ####p < 0.0001 by two-way ANOVA with Dunnett's post hoc testing. Asterisks indicate significance compared to HOCl treatment alone, and # indicates significance between the immediate analysis data and the 24 h re-incubation data.