Figure 2.

Verification of candidate ncRNA in SCI rat model. (A, B) Evaluation of rat SCI model construction process. (A) Before and after the rat model was established, the BBB score was used to evaluate the exercise ability of the rats. No. 1 to No. 5 are control group and No. 6 to No. 10 are SCI model group. (B) 7 days after the rat SCI model was established, rat spinal cord tissue was collected and analyzed by HE staining. All HE stained pictures were magnified 200 times. (C-E) Seven days after the rat SCI model was established, total RNA from rat spinal cord tissue was reverse transcripted with specific primers, and analyzed by qPCR. The expression level of predicted up-regulated miRNA (C), down-regulated miRNA (D) and lncRNA-MEG (E) are normalized to U6. Each data represented the mean ± SD of three independent experiments and were analyzed with T-test. *P < 0.05, **P < 0.01, ***P < 0.001.